Figure 8

Prediction of the structural relationship between the wheat SSs and query proteins with the 3D structure of Agrobacterium tumefacien glycogen synthase. The predicted amino acid sequence of 32 SSs from wheat, rice, Arabidopsis, Chlamydomonas and Ostreococcus and two GSs from Synechocystis were compared to AgtGS. Sequences were aligned using Clustal W [34]. The alignment was manually adjusted using secondary structure predictions. The key is as follows: regions predicted to form α-helices – grey rectangles; black arrows – β-sheets. Conserved amino acid residues are highlighted. The two major Loops, the 380s Loop or stretch of polypeptides (labeled), as well as the second Loop in Motif VI (which is highlighted in green), are indicated.