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Figure 6 | BMC Plant Biology

Figure 6

From: Cloning, characterisation and comparative analysis of a starch synthase IV gene in wheat: functional and evolutionary implications

Figure 6

Transcript analysis of the wheat SSs in leaf by comparative RT-PCR under varying conditions of light. A. Transcript levels of TaSSIVb, TaSSIIIa, TaGBSSIb and TaGAPDH during the course of the photoperiod in wheat leaves as determined using comparative RT-PCR. RNA was extracted from leaves sampled from 14-day old wheat plants grown under a regime of 16 h light and 8 h dark in a greenhouse. Open bars correspond to the 16 h-light period; the solid bars correspond to the 8 h-dark period. Leaves were harvested at the end of the dark/beginning of the light period (1), the middle of the light period (2) and in the middle of the dark period (3). Results are typical of three experiments. B. Transcript levels of the TaSSIVb, TaSSIIIa, TaGBSSIb and TaGAPDH during an alternating 24 h-dark and light period. Plants were grown as described in A but were transferred to a growth chamber and acclimated to 16:8 light:dark regime for 2 days. RNA was extracted from leaves of eight 14-day old plants after 16 h light (and 0 h dark) (1), 4 h (2), 16 h (3) and 24 h (4) in the dark, followed by 4 h (5), 16 h (6) and 24 h (7) in the light. Solid bars correspond to the dark period; the white stippled areas correspond to what in a 16:8 cycle would have been the light period. Open bars correspond to the light period; the stippled area corresponds to what in a 16:8 cycle would have been the dark period.

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