Figure 12

An alignment showing the position of introns relative to amino acid sequence within SS classes. An alignment was made with the SS protein sequences by BLASTP [64] and this was compared to the genomic sequence of each protein in turn. The presence of an intron at the position of an amino acid in the alignment is illustrated by a "1" when the splicing occurs inside the amino acid or by "1+" when the splicing occurs just after the amino acid. When an intron was positioned at the same amino acid in all of the sequences of a particular SS subfamily in the alignment, this was illustrated in the figure by color-filled squares. Introns were identified by comparing the cDNA against the genomic sequence of each SS using Spidey at NCBI [75] and were manually checked for non-canonical sites. Genbank Accession numbers ascribed to the nucleotide sequences are as follows: AtGBSSI [AT1G32900.1A], TaGBSSIa [AB019624], AtSSI [AF121673]; TaSSI [AF091802]; AtSSII [AC008261]; TaSSIIa [AY133248]. Genbank Accession numbers for the nucleotide sequences of other SSs and amino acid sequences are given elsewhere.