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Table 3 Leaf starch properties

From: Overlapping functions of the starch synthases SSII and SSIII in amylopectin biosynthesis in Arabidopsis

Genotype Amylose content Amylopectin λmax Phosphate
  % (mg/g FW)   
Wild typea 21.6 ± 1.4 (5) 1.20 545 nm 4.35 ± 0.43 (2)
Atss3-1 a 20.7 1.40 555 nm 10.7 ± 0.03 (2)
Atss2-1 a 33.7 1.75 565 nm 3.01 ± 0.24 (2)
Atss2-2 a 34.2 2.11 565 nm NA
Atss2,Atss3a 40.1 0.63 575 nm NA
Wild typeb 25.2 ± 1.8 (5) 2.17 549 nm NA
Atss2-3 b 42.7 ± 6.7 (5)c 2.99 555 nm NA
  1. aColumbia genetic background
  2. bWS genetic background
  3. cValue significantly different from wild type as indicated by P-value < 0.05.
  4. Starch was analyzed from leaves harvested at the end of the light period of the LD diurnal cycle. GPC fractions 42 and lower were designated as amylopectin and fractions 43 and higher were designated as amylose (Figure 7). Parentheses indicate the number of independent biological replicate assays used to generate the mean and standard deviation. The Atss2-1 and Atss2-2 mutant lines were analyzed only once, however, these assays are independent biological replicates for the condition in which SSII is absent. Amylose content was normalized to leaf fresh weight by multiplying the normalized total starch amount from Table 3 by the amylose percentage determined for each genotype. Phosphate content units are nmol phosphoryl groups/mg starch. "NA" indicates data not available. Data for Atss3-1 were previously published [29] in a study using identical analytical methodology.