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Table 1 Starch metabolic enzyme activities in soluble leaf extracts

From: Overlapping functions of the starch synthases SSII and SSIII in amylopectin biosynthesis in Arabidopsis

Enzyme Activity by genotype
  Wild type Atss2-1 Atss3-1 Atss2/Atss3
SSa 212 ± 8.66 (4) 237 ± 8.74 (4) 265 ± 18.6 (4) 190 ± 8.78 (4)
SS 9.24 ± 0.80 (2) 9.19 ± 0.16 (2) 9.82 ± 0.07 (2) 9.85 ± 0.41 (2)
SBEb 50.3 ± 7 (1) 52.6 ± 9.9 (1) 45.8 ± 5.7 (1) 51.5 ± 10.0 (1)
ADPGPP 15.5 ± 3.8 (3) 19.9 ± 3.1 (3) 14.5 ± 2.4 (3) 14.5 ± 2.5 (3)
ADPGPP + 3PGA 35.3 ± 1.8 (3) 33.7 ± 4.0 (3) 22.1 ± 6.9 (3) 23.2 ± 13.0 (3)
α-amylase 32.6 ± 2.0 (3) 33.7 ± 3.4 (3) 35.5 ± 1.3 (3) 44.1 ± 3.7 (3)
β-amylase 191 ± 27 (3) 237 ± 46 (3) 259 ± 22 (3) 243 ± 30 (3)
Pullulanase-type DBE 8.40 ± 1.7 (3) 15.4 ± 1.8 (3) 2.06 ± 0.34 (3)c 3.90 ± 1.9 (3)
DE 25.6 ± 5.8 (3) 29.3 ± 3.0 (3) 30.4 ± 0.65 (3) 32.8 ± 1.8 (3)
Maltase 42.5 ± 4.7 (3) 37.6 ± 0.59 (3) 40.5 ± 4.9 (3) 38.5 ± 9.8 (3)
SPb 1.34 ± 0.20 (1) 2.86 ± 0.22 (1) 0.70 ± 0.35 (1) 0.97 ± 0.08 (1)
  1. Unless otherwise noted, units are nmol product/min/mg protein. Values in parentheses indicate the number of independent biological replicates used to generate the mean and standard error values indicated.
  2. Abbreviations are as follows: DE: disproportionating enzyme; SP, starch phosphorylase; ADPGPP: ADPGlc pyrophosphorylase; DBE: starch debranching enzyme; 3PGA: 3-phosphoglycerate.
  3. a SS activity was measured independently in two completely separate sets of assays. For the assays in this row only the units are nmol glucose incorporated/g FW/min.
  4. b The values for SP and BE activity were determined by triplicate measurements from a single plant extract.
  5. c Value is significantly different from wild type after Dunnet's adjustment for multiple comparisons. Unless so indicated, values are not significantly different from wild type.