Figure 7
Glucan separation by gel permeation chromatography. A, Wild type and Atss2-1. Purified granules from plants of the indicated genotypes were dissolved by boiling in DMSO, and the polymers present were separated by GPC on Sepaharose CL-2B on a column measuring 1.8 cm i.d. × 1 m height. Glucan polymer in each fraction was enzymatically quantified, and samples of each fraction were stained with I2/KI solution. Visible absorbance spectra were recorded and the maximal absorbance wavelength is noted. B, Wild type and Atss2, Atss3. Analysis was as in panel A, except that the column size was 1.2 cm i.d. × 50 cm height.