Glucan content. A, Iodine staining. Leaves were harvested at the end of the light phase from plants of the indicated genotype grown for several weeks in long day growth room conditions. These leaves were then decolorized, stained with I2/KI solution, and destained for 5 – 10 min in water. B, Starch quantification. Starch was isolated from leaves of plants grown under long day growth room conditions, harvested at specific times in the 16 h light/8 h dark diurnal cycle. Granules were collected by low-speed centrifugation and the glucan content in the pellet quantified. Each point is the average of leaves from six individual plants, and standard error is indicated.