Figure 4

Example of localization of ISBP markers onchromosome arm 1RS. PCR products obtained after amplification with two ISBP primer pairs were visualized on a 2% agarose gel. Templates were DNA of rye (1), wheat (2), wheat-rye 1RS addition (3), flow-sorted chromosome 1R (4), and flow-sorted chromosomes 2R – 7R (5) were used for testing marker specificity. BAC pools of plate SccImp1RShA_0079 (6), SccImp1RShA_0127 (7), SccImp1RSbA_0175 (8), and SccImp1RSbA_0223 served to estimate frequency of ISBP sites on 1RS and as positive control. Both markers were positive in rye, the wheat-rye 1RS addition line and in flow-sorted chromosome 1R and negative in wheat and flow-sorted chromosomes 2R – 7R and thus were considered 1RS specific. M – 100 bp DNA ladder.