Survey of eccDNA derived from various plant satellites. Samples of genomic DNA enriched for circular DNA were separated on 0.7% and 2% 2-D gels, blotted and hybridized with satellite-specific probes. The arcs corresponding to linear (L) and open circle (OC) DNA are indicated with black arrowheads and positions of the longest (1,674 bp) and the shortest (558 bp) OC markers are shown as blue spots. The extra arc of unknown origin is indicated with black arrow on panels A, B, D and E. (A-B) Detection of Sc119 repeats in Secale cereale, demonstrating the effect of single-stranded DNA degradation by mung bean nuclease (B) compared to untreated control (A). Figure insets contain magnified regions marked by the rectangles showing the effect of the nuclease treatment on shape of the spots. All other samples shown on panels C-L were also treated with mung bean nuclease. (C) Afa repeats in Triticum aestivum, (D) IGS-like in Vicia sativa, (E) CentO in Oryza sativa, (F) FokI in V. faba, (G) PisTR-B in Pisum sativum (this sample was resolved on 1.5% and 2% 2-D gels), (H) VicTR-B in V. grandiflora, (I) VG-V subfamily of VicTR-B in V. grandiflora, (J) VicTR-A in V. pannonica detected using PCR probe including a mixture of genomic VicTR-A sequences, (K-L) VicTR-A in V. pannonica detected using specific clones c653 (K) and c666 (L).