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Table 1 Kinetic properties of Rubisco purified from Chlamydomonas wild type, mutant D470P/T471A/I472M/K474T, and spinach

From: Plant-like substitutions in the large-subunit carboxy terminus of Chlamydomonas Rubisco increase CO2/O2 Specificity

Kinetic constant

Wild type

Mutant

Spinach

Ωa (VcKo/VoKc)

61 ± 1

67 ± 2

81 ± 3

Vc a (μmol/h/mg)

119 ± 11

100 ± 7

63 ± 6

k cat b (s-1)

2.7 ± 0.2

2.3 ± 0.2

1.4 ± 0.1

Kc a (μM CO2)

34 ± 2

40 ± 5

21 ± 1

Ko a (μM O2)

417 ± 62

611 ± 119

496 ± 40

Vc/Kc b

3.5

2.5

3.0

Ko/Kc b

12

15

24

Vc/Vo b

5.1

4.5

3.4

  1. a Values are the mean ± (n-1) standard deviation of three or more separate enzyme preparations. b Calculated values.
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BMC Plant Biology

ISSN: 1471-2229

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