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Figure 3 | BMC Plant Biology

Figure 3

From: Plant-like substitutions in the large-subunit carboxy terminus of Chlamydomonas Rubisco increase CO2/O2 Specificity

Figure 3

Carboxy-terminal substitutions do not influence the structural stability of the enzyme in assays performed in vitro. Thermal inactivation of Rubisco purified from wild type () and the D470P/T471A/I472M/K474T quadruple mutant (). Rubisco enzymes were incubated at each temperature for 10 min, and then assayed for RuBP carboxylase activity at 25°C. Activities were normalized to the specific activities measured after the 35°C incubation (wild type, 1.7 μmol/min/mg; D470P/T471A/I472M/K474T, 1.2 μmol/min/mg).

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