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Table 2 Predicted rice GH family 35 β-galactosidase protein properties and locations.

From: Genomic and expression analysis of glycosyl hydrolase family 35 genes from rice (Oryza sativa L.)

Gene Name

Gene Locus

(NCBI, RGP)

Pre-protein

Mature Protein

  

MWa (kDa)

AAb

Cleavage sitec

MWa

AAb

pIa

N-gly sited

Possible destinatione

OsBgal1

Os03g0165400

92.2

841

25–26

89.8

816

5.78

2

Gol, Vac, ER ext

OsBgal2

Os06g0573600

78.3

715

20–21

76.5

695

7.65

0

Gol, PM, IMP, MII, ER ext

OsBgal3

Os01g0580200

91.5

827

24–25

89.5

803

5.59

2

Gol, PM, IMP, MII, ER, Sec

OsBgal4

Os01g0875500

94.7

851

29–30

91.7

822

6.76

6

Gol, Cis-Gol, MII, ER, Sec

OsBgal5

Os01g0533400

91.7

827

25–26

88.9

802

5.66

8

Gol, MII, ER

OsBgal6

P0636F09.15

90.6

811

17–18

88.9

794

6.61

5

IMP, MC in, Sec, Cy, N

OsBgal7

Os02g0219200

78

729

36–37

76.8

693

8.71

0

Gol, MII, ER, Sec

OsBgal8

Os03g0255100

103.7

956

62–63

96.5

894

5.62

7

PM, IMP, ER, Sec

OsBgal9

Os05g0539400

75.7

673

20–21

73.5

653

5.57

6

Vac-Lys, IMP, MC in, Per, ERl, N, Sec, Cy

OsBgal10

Os08g0549200

94.7

848

23–24

92.8

825

9.1

5

Vac-Lys, PM, IMP, MC in, ER, Cy, Sec

OsBgal11

Os09g0539200

94.11

838

25–26

91.9

813

6.52

3

Vac-Lys, Gol, PM, IMP, MII, Mic, ER, MC in, Sec

OsBgal12

Os10g0330600

92

828

23–24

89.7

805

6.07

10

Gol, MII, ER, Sec

OsBgal13

Os12g0429200

101.0

919

31–32

97.9

888

5.56

5

IMP, ER, Sec

OsBgal14

J090043H02

92.2

828

28–29

89.6

800

5.99

9

PM, IMP, MII, ER, Sec

OsBgal15

AP004733

89.6

809

23–24

87.3

789

6.17

8

Gol, MII, ER, Sec

  1. a determined by ProtParam. b AA means number of amino acids. cpredicted by SignalP [35]. dpredicted by NetNGlyc at the Expasy proteomics server http://www.expasy.org and manually curated to remove NPS/T sites, ecellular locations predicted by PSORT [65], abbreviated as PM: plasma membrane, MII: Type II membrane protein, Gol: Golgi, IMP: Integral Membrane Protein, Sec: Secreted, ER: Endoplasmic reticulum; ext: extracellular; l: lumen, MC: Mitochondrial; in: inner membrane, Cy: Cytoplasmic, N: nuclear, Vac: vacuoles, Vac-Lys: Lysosome-like vacuoles, Per: Peroxisomal, and Mic: microsomal.