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Figure 3 | BMC Plant Biology

Figure 3

From: Quantitative analysis of changes in actin microfilament contribution to cell plate development in plant cytokinesis

Figure 3

Effects of bistheonellide A (BA) on the duration required to complete cytokinesis. (A) Time-lapse images of FM4-64-labelled cell plate expansion in control cells. Time 0 min represents chromosomal separation. Yellow broken lines indicate parental cell wall. (B) Effect of BA treatment on MFs in cytokinetic BY-2 cells. BY-GF11 cells were treated with DMSO or 1 μM BA for 1 h. Representative images of a single optical section at the mid-plane (mid-plane) and maximum intensity projection (projection) are presented. (C) Time-lapse images of FM4-64-labelled cell plate expansion in BA-treated cells. Time 0 min represents chromosomal separation. Yellow broken lines indicate parental cell wall. (D) Effect of BA treatment on the duration between chromosomal separation and complete fusion of a cell plate and parental cell wall. Note that the duration times in BA-treated cells were longer than those in control cells, and were independent of cell size. The data were obtained from 12 independent experiments of each condition. Scale bars indicate 10 μm.

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