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Figure 2 | BMC Plant Biology

Figure 2

From: Sgt1, but not Rar1, is essential for the RB-mediated broad-spectrum resistance to potato late blight

Figure 2

Transcription analysis of Rar1 and Sgt1 silenced transgenic lines. (A) Northern blot analysis of transcription of the Rar1 gene. Lane 1: a transgenic plant (clone 3007) containing the Rar1-RNAi construct but not silenced; Lane 2–4: three independent Rar1-silenced lines (clones 3128, 2998, 3028) showing no transcript; Lane 5: untransformed K41 control. (B) Northern blot analysis of transcription of the Sgt1 gene. Lane 1: a transgenic plant (clone 3061) containing the Sgt1-RNAi construct but not silenced; Lane 2: Sgt1-silenced line (clone 3095) showing no transcript; Lane 3: untransformed K41 control. Both blots were re-probed with an actin gene probe to ensure equal loading of mRNA in each lane.

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