ARR22 specifically interacts with AHP2, 3 and 5 in tobacco leaf cells. (A) Confocal images of abaxial epidermal tobacco leaf cells expressing the indicated YFP-N and YFP-C fusion proteins (left column). The right column shows the corresponding bright field images of the transformed cells. The bars represent 25 μm. (B) Western blot analysis of protein extracts derived from transiently transformed tobacco leaves assayed for BiFC fluorescence before extraction (1–6). Immunodetection of the YFP-N fusion proteins (AHPs) was carried out with an antibody against c-myc-tag (anti c-myc) and of the YFP-C fusion protein (ARR22) with an antibody against the HA-tag (anti HA). M, protein marker.