ARR22 is predominantly localized in the cytoplasm of tobacco leaf cells. Confocal images of abaxial epidermal leaf cells expressing the indicated RFP and GFP fusion proteins are shown. The emission channels for the RFP (ARR22:RFP) and GFP (marker fusion proteins) fluorescence and the overlay are indicated at the left. The lowest row shows the bright field images of the transformed cells. Marker genes are ARR2 for the nucleus, AHK5 for the cytoplasm and plasmalemma and GFP for nucleo-cytoplasmic distribution. The white arrows indicate the dense cytoplasm around the nucleus. The bars represent 10 μm.