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Table 1 Genes from 22 k Arabidopsis ATH1 array that have the highest correlation across 965 chips with the hub genes.

From: Articulation of three core metabolic processes in Arabidopsis: Fatty acid biosynthesis, leucine catabolism and starch metabolism

Hub Gene: MCCB, At4g34030, methylcrotonyl-CoA carboxylase, subunit B, leucine catabolism

Corr.

GeneID

Description

0.9

At1g03090

MCCA, methylcrotonyl-CoA carboxylase, subunit A

0.84

At3g06850

BCE2, E2 subunit of branched chain alpha-ketoacid dehydrogenase

0.84

At3g13450

DIN4, E1 beta subunit of branched-chain alpha-keto acid dehydrogenase

0.82

At2g43400

electron transfer flavoprotein: ubiquinone oxidoreductase family protein

0.8

At3g45300

IVD, isovaleryl-CoA dehydrogenase

0.8

At4g35770

Senescence-associated protein (SEN1)

0.79

At5g21170

AMP-activated protein kinase

0.78

At1g08630

threonine aldolase

0.78

At1g15040

glutamine amidotransferase-related

0.77

At1g10070

BCAT2, branched-chain amino acid transaminase

0.74

At4g28040

nodulin MtN21 family protein

0.73

At3g47340

glutamine-dependent asparagine synthetase 1 (ASN1)

0.73

At1g21400

E1 alpha subunit of branched-chain alpha-keto acid dehydrogenase, BCDH_E1a2

0.72

At1g18270

ketose-bisphosphate aldolase class-II family protein

0.71

At5g49450*

bZIP family transcription factor

0.71

At1g55510

BCDH BETA1, E1 beta subunit of branched-chain alpha-keto acid dehydrogenase

0.7

At3g49790

expressed protein

Hub Gene: DE2, At2g40840, disproportionating enzyme, starch metabolism

Corr.

GeneID

Description

0.89

At3g46970

PHS2, starch phosphorylase

0.85

At1g10760

GWD1, glucan water dikinase

0.85

At3g52180*

SEX4, protein tyrosine phosphatase/kinase

0.8

At1g03310

ISA2, isoamylase DB

0.79

At4g09020

ISA3, isoamylase DB

0.76

At1g06460

31.2 kDa small heat shock family protein/hsp20 family protein

0.74

At5g26570

GWD2, glucan water dikinase

0.74

At3g29320

PHS1, starch phosphorylase

0.73

At5g59130

subtilase family protein

0.73

At2g39920

acid phosphatase class B family protein

0.72

At1g69830

AAM3, alpha-amylase

0.72

At1g67660

expressed protein

0.71

At3g18500

similar to endonuclease/exonuclease/phosphatase family protein

0.7

At2g31040

ATP synthase protein I-related

0.7

At4g33490

nucellin-like protein

Hub Gene: MOD1, At2g05990, enoyl reductase, fatty acid biosynthesis

Corr.

GeneID

Description

0.86

At1g34430

PDHC_E2, acetyltransferase subunit of pyruvate dehydrogenase

0.86

At5g10160

HD, 3-hydroxyacyl-[ACP] dehydratase

0.84

At5g35360

CAC2, biotin carboxylase subunit of heteromeric acetyl-CoA carboxylase

0.83

At5g46290

KASI, 3-ketoacyl-[ACP] synthase I

0.8

At2g30200

MAT, malonyl-CoA:ACP transacylase

0.8

At3g16950

LPD1, lipoamide dehydrogenase subunit of pyruvate dehydrogenase

0.79

At1g62640

KAS III, 3-ketoacyl-[ACP] synthase III

0.78

At1g53520$

chalcone-flavanone isomerase-related

0.77

At3g25860

LTA2, acetyltransferase subunit of pyruvate dehydrogenase

0.77

At5g52920

pyruvate kinase

0.76

At5g16390

BCCP1, biotin carboxyl carrier subunit of heteromeric acetyl-CoA carboxylase

0.75

At5g08415

LIP1-like, lipoate synthase

0.73

At2g22230

HD-like, 3-hydroxyacyl-[ACP] dehydratase

0.72

At1g30120

PDHC_E1beta2, E1 beta subunit of plastidic pyruvate dehydrogenase

0.72

At5g15530

BCCP2, biotin carboxyl carrier subunit of heteromeric acetyl-CoA carboxylase

0.71

At1g01090

PDHC_E1alpha, E1 alpha subunit of plastidic pyruvate dehydrogenase

0.71

At5g50390*

pentatricopeptide (PPR) repeat-containing protein

0.7

At4g12700

expressed protein

  1. Genes used as the hub (at the top of the table) are methylcrotonyl-CoA carboxylase (MCCB), active in leucine degradation in mitochondria; disproportionating enzyme (DE2), from starch metabolism; and enoyl reductase (MOD1), an enzyme from plastidic fatty acid biosynthesis pathway. Genes that code for proteins predicted to be in the same pathway as the hub gene are in bold font. Potential regulatory genes are marked with an asterisk. Pearson coefficients (> 0.7) were calculated by MetaOmGraph. P-values for enrichment of genes from same pathway as hub are 4.35e-36, 2.02e-15, and 3.1e-23, respectively.
  2. $ This gene product, although annotated as chalcone isomerase-like, appears to have a direct role in binding and possibly transporting fatty acids (Joe Noel and Florence Pojer, personal communication).