Figure 4

Coexpression of the genes for fatty acid biosynthesis. A – Fatty acid biosynthesis in chloroplast. Candidate genes for enzymatic function (enzyme names in blue) are shown in black or red font; genes whose Locus ID is in red are co-expressed. These genes form connected network in Fig. 2B and encode all enzymes required for the biosynthesis of 18-carbon fatty acids from acetyl-CoA (sequence of reactions on the lighter green background), as well as genes for producing of acetyl-CoA substrate, and for biosynthesis of biotin and lipoic acid, obligate cofactors for the acetyl-CoA carboxylase and for pyruvate dehydrogenase (sequence of reactions on the lighter yellow background). Gene for pyruvate kinase (on yellow background, identified by search for genes co-expressed with MOD1), required for production of pyruvate, is also co-expressed with genes from the fatty acid biosynthesis pathway [see Additional file 1 for the correspondence between gene names and Locus IDs]. B – Expression profiles of the 11 most tightly co-expressed genes from fatty acid biosynthesis module across 956 microarray chips (top panel) and, for comparison, expression profiles of the 11 randomly chosen genes (bottom panel). Experiments with tissues and organ samples are denoted: WP, whole plant; RL, rosette leaf; F, fruit; R, root; S, shoot; SD, seed; L, leaf; FL, flower; G, male gametophyte; H, hypocotyls; LA, leaf apex; O, other. ABC, acyl transporter; ACC, heteromeric acetyl-CoA carboxylase; ACS, acetyl-CoA synthetase; BIO2, biotin synthase; ENR, enoyl-[ACP] reductase; FAD, fatty acyl-[ACP] desaturase; FAT, fatty acyl-[ACP] thioesterase; HD, 3-hydroxyacyl-[ACP] dehydratase; KAR, 3-ketoacyl – [ACP] reductase; KAS, 3-ketoacyl-[ACP] synthase; LACS, plastidial long-chain acyl-CoA synthetase; lipS, lipoate synthase; lipT, lipoate transferase; MAT, malonyl-CoA:ACP transacylase; PDHC, pyruvate dehydrogenase complex; PK, pyruvate kinase.