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Figure 5 | BMC Plant Biology

Figure 5

From: Head-neck domain of Arabidopsis myosin XI, MYA2, fused with GFP produces F-actin patterns that coincide with fast organelle streaming in different plant cells

Figure 5

F-actin-binding of GFP-MYA2-head6IQ and RFP-FABD2 in respect to microfilament subpopulations and peroxisome pathways in different plant cells. (A-I) Co-expression of fusion proteins in A. cepa: GFP-MYA2-head6IQ (A) with RFP-FABD2 (B), YFP-mTn (D) with RFP-FABD2 (E) and GFP-MYA2-head6IQ (G) with a peroxisomal marker fused with mcherry (H). Merged images are depicted in (C), (F) and (I), respectively. (J-L) Two-sec-interval images of an epidermal cell of Sinapis cotyledons co-transformed with GFP-MYA2-head6IQ (GFP in green) and a peroxisomal marker (mcherry in red). (M) Co-transformation of a cell of A. cepa with RFP-FABD2 (RFP in red) and a peroxisomal marker (CFP in green). Widefield fluorescence images were taken at 15 h (A-I) or 18 h (J-M) after transformation. Bar in (I) = 5 μm for all images in the upper panel. Bars in (J-M) = 10 μm.

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BMC Plant Biology

ISSN: 1471-2229

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