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Figure 3 | BMC Plant Biology

Figure 3

From: Head-neck domain of Arabidopsis myosin XI, MYA2, fused with GFP produces F-actin patterns that coincide with fast organelle streaming in different plant cells

Figure 3

Localization of GFP-MYA2-head6IQ and F-actin by GFP fluorescence in different plant cells. (A-C, G, I) F-actin-visualization by GFP-MYA2-head6IQ. For comparison, F-actin is visualized by RFP-FABD2 (D, H, J) and GFP-FABD2 (E) or by YFP-mTn (F). The panels in (G) and (H) show the distribution of F-actin in cells at three different depths. The nuclear localization of GFP-MYA2-head6IQ and RFP-FABD2 signals is shown in panels (I) and (J). Images in (I*, J*) and (I**, J**) derive from optical sections through the center of the cell nucleus and its periphery, respectively. Expression times were 6–7 h for (A) and 15 h for all other images except for 5 d for (C, F). All images represent epidermal cells of A. cepa except for (C) and (F) which represent leaf epidermal cells of N. benthamiana. Bars in I, J = 5 μm. Bars in all other images = 15 μm.

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BMC Plant Biology

ISSN: 1471-2229

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