Figure 7
From: Roles of ArabidopsisWRKY3 and WRKY4 Transcription Factors in Plant Responses to Pathogens
Responses of wrky4 mutants and transgenic WRKY4 -overexpressing plants to Pst DC3000. (A) Altered bacterial growth in the WRKY mutants. Wild type (WT), wrky4 mutants and WRKY4-overexpressing lines 7 and 13 (35S:W4-L7 and 35S:W4-L13) were infiltrated with a suspension of PstDC3000 (OD600 = 0.0001 in 10 mM MgCl2). Samples were taken at 0 (open bars) or 3 days (closed bars) post inoculation (dpi) to determine the growth of the bacterial pathogen. The means and standard errors colony-forming units (cfu) were calculated from 10 plants for each treatment. (B) Disease symptom development. Pathogen inoculation of wild type, mutants and overexpression lines was performed as in A. Pictures of representative inoculated leaves taken at 4 dpi. (C) Pathogen-induced PR1 expression. Wild type and WRKY4-overexpressing plants were infiltrated with a suspension of PstDC3000 (OD600 = 0.0001 in 10 mM MgCl2). Inoculated leaves were collected at indicated dpi for RNA isolation. RNA gel blot analysis was performed with 32P-labeled PR1. These experiments were repeated three times with similar results.