Figure 4

Indirect immunofluorescence detection of xyloglucan epitopes in sections of tamarind and nasturtium seed cotyledon parenchyma. a. LM15 binding to cell walls of tamarind cotyledon parenchyma cells. b. Same section stained with Calcofluor White showing extent of cell walls. c. LM15 binding to cell walls of nasturtium cotyledon parenchyma cells. d. Micrograph c combined with Calcofluor White fluorescence. e. CCRCM1 binding to cell walls of nasturtium cotyledon parenchyma cells. f. Micrograph e combined with Calcofluor White fluorescence. Arrowheads in a and b indicate inner cell wall. Arrowheads in c and d indicate inner edge of abundant LM15 immunolabelling. Arrowheads in e and f indicate inner cell wall and region of CCRCM1 immunolabelling. Scale = 20 μm.