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Table 1 Expenditure of time for image acquisition

From: A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs

Action 1st pass: preview scan (5×-objective, 432 × 342 pixels) 2nd pass: detailed scan (10×-objective, 1300 × 1030 pixels)
stage positioning ≈ 0.5 s ≈ 1 s
auto focus negligible (once per slide) ≈ 3 s
single shot ≈ 0.7 s ≈ 1.5 s
several focal layers -- 5 layers per position
time expenditure 10 min per slide (scanning 15 × 38 = 570 positions) 20 min per slide (scanning ≈ 100 positions*)
  1. *A number of 100 positions is an average, depending on the actual quantity of transformed cells on the slide.