Figure 3

Localisation of root hair formation is altered in the prc1-1 mutant. The positions of root hairs relative to the basal cell wall were measured for wild-type and prc1-1 seedlings. (A) Wild-type showing typical root hair emergence towards the basal end of the trichoblast. (B) pcr1-1 showing a basally hyperpolarized root hair. (C) prc1-1 showing an apically shifted root hair. (D) Frequency distribution of relative root hair position for wild-type and prc1-1. The Rop protein was localized in wild-type and prc1-1 roots using a specific anti-Rop antibody and DAPI staining was used to highlight the position of the nucleus. The Rop signal is shown in green and DAPI in blue (E) wild-type (F) basally hyperpolarised Rop signal in prc1-1 (G) apically shifted Rop signal in prc1-1 trichoblast. Arrowheads indicate the location of lateral cell-cell interfaces. Bar = 50 μm (A, B, C) 20 μm (E, F, G).