Comparative expression profiling in grape (Vitis vinifera) berries derived from frequency analysis of ESTs and MPSS signatures

Table 5 Most highly expressed grape EST contigs in the grape berry stage II libraries, based on MPSS signature abundance.

Contig ID	Signature abundance^a	ESTs^b	ESTs^c	Total ESTs^d	BlastX definition [species of closest EST match]
1027142, 1026885, 1027113	108049	31	81	112	2S albumin precursor [Vitis vinifera]
1027101	67435	52	66	118	AF449424_1 11S globulin-like protein [Corylus avellana]
1027135, 1027103, 1027117	60308	17	36	53	Storage protein [Corylus avellana, Coffea arabica and Prunus dulcis]
1027226	46226	49	31	80	conglutin gamma [Lupinus albus]
1027222	18313	34	8	42	AF240006_1 7S globulin [Sesamum indicum]
1027379, 1026935	17310	7	8	15	plasma membrane aquaporins
1027444, 1027445, 1027446	13927	185	187	372	Endochitinase and class Ib chitinase [Galega orientalis]
1027477	13748	2	4	6	hexameric polyubiquitin
1027684, 1027685	10450	3	17	20	expressed protein [Arabidopsis thaliana]
1027454	6505	12	7	19	AF121261_1 elongation factor 1-alpha 1; EF-1-alpha1 [Lilium longiflorum]
1027543, 1027547, 1027548, 1027553, 1027554	5994	3	14	17	T06239 probable glutathione transferase (EC 2.5.1.18), 2,4-D inducible – soybean
1026907	5460	4		4	T10174 hypothetical protein – castor bean
1027486	5403	3	2	5	hexameric polyubiquitin
1027455	4782		1	1	AF121261_1 elongation factor 1-alpha 1; EF-1-alpha1 [Lilium longiflorum]
1027495	4688	2	4	6	ARF_ORYSA ADP-ribosylation factor
1027181	4668	2	1	3	TCTP_HEVBR Translationally controlled tumor protein homolog (TCTP)
1026972, 1027353, 1027133	4642	14	28	42	metallothionein-like protein type 2 [Persea americana]
1027119	4419	1	6	7	ATFP3 [Arabidopsis thaliana]
1027883	4390	1		1	LTCOR11 [Lavatera thuringiaca]
1026987	4208	36	22	58	AF281656_1 putative transcription factor [Vitis vinifera]

The expression level was determined based on the signature with the maximum normalized abundance.
^a The sum of abundance of matching 17-base MPSS signatures from the grape library.
^b ESTs derived from stage II green, hard berries
^c ESTs derived from stage II green, soft berries
^d Total signatures matching contigs present in the UC Davis berry stage II libraries.

ISSN: 1471-2229