Comparative expression profiling in grape (Vitis vinifera) berries derived from frequency analysis of ESTs and MPSS signatures

Table 1 Summary statistics of raw 17- and 20-base MPSS signatures from grape berries.

	17-base signatures		20-base signatures
A. Summary of sequencing results.
Sequencing frame	Absolute	% of Total	Absolute	% of Total
2-step run	1,194,288	45.3%	1,002,346	44.4%
4-step run	1,441,005	54.7%	1,256,940	55.6%
B. Single filter results.
	17-base signatures		20-base signatures
Reliability Filter ^a	Count	Proportion	Count	Proportion
R	17,976	58.5%	15,699	58.4%
nR	12,761	41.5%	11,179	41.6%
Significance Filter ^a
S	16,029	52.1%	13,817	51.4%
nS	14,708	47.9%	13,061	48.6%
C. Combined filter results.
	17-base Total		17-base Distinct		20-base Total		20-base Distinct
Filter categories ^a	Absolute	% of Total	Count	% of Total	Absolute	% of Total	Count	% of Total
RS	2,550,024	96.8%	13,586	44.2%	2,187,369	96.8%	11,874	44.2%
RnS	17,862	0.7%	4,390	14.3%	14,958	0.7%	3,825	14.2%
nRS	47,346	1.7%	2443	7.9%	39,338	1.7%	1943	7.2%
nRnS	20,061	0.8%	10,318	33.6%	17,621	0.8%	9,236	34.4%
TOTAL	2,635,293	100.0%	30,737	100.0%	2,259,286	100.0%	26,878	100.0%

"Absolute" indicates the total number of successful sequencing reads, while "count" or "distinct" indicates the number of different sequences.
^a R = reliable, nR = non-reliable, S = significant, nS = non-significant, RS = reliable and significant, RnS = reliable and non-significant, nRS = non-reliable and significant, nRnS = non-reliable and non-significant.

ISSN: 1471-2229