A flax fibre proteome: identification of proteins enriched in bast fibres

BMC Plant Biology

Table 2 Experimental design relative to labeling and sample loading of analytical gels.

gel	Cy2 labeled	Cy3 labeled	Cy5 labeled
1	internal standard #1 30 μg	fibre sample #1 30 μg	non-fibre sample #1 30 μg
2	internal standard #2 30 μg	fibre sample #2 30 μg	non-fibre sample #2 30 μg
3	internal standard #3 30 μg	non-fibre sample #3 30 μg	fibre sample #3 30 μg
4	internal standard #4 30 μg	non-fibre sample #4 30 μg	fibre sample #4 30 μg

Note: each gel contains proteins from a unique pool (#1–#4) of independently grown plants. The Cy2-labeled internal standard is a mixture of equal masses of proteins from fibre and non-fibre samples.

ISSN: 1471-2229