Table 3 Details of the newly developed SSR primers
Sl. No. | Primer Id | Primer sequence (F: Forward; R: reverse) | Repeat unit | Ta (°C) | Amplicon (bp) | GenBank accession No. | Linkage group |
|---|---|---|---|---|---|---|---|
1 | CaM02 | F: CGCCAGCCACAGCCACTTGC | (AGG)7 | 50 | 224 | EU526557 | -- |
| Â | Â | R: GCGGGGGTAAGAAAGAGGCGAG | Â | Â | Â | Â | Â |
2 | CaM03 | F: CGCGCTTGCTCCCTCTGTCTCT | (AC)11 | 57 | 173 | EU526558 | CLG03 |
| Â | Â | R: TGGGGGAGGGGCGGTGTT | Â | Â | Â | Â | Â |
3 | CaM06 | F: ACCCGATATTCAACCGACATGC | (CT)7 | 50 | 278 | EU526559 | -- |
| Â | Â | R: CATGACTTGAGCGCTAATATTTGAT | Â | Â | Â | Â | Â |
4 | CaM08 | F: CAGCTGAAGTGGTGAAAAACAAGAG | (TC)8 | 50 | 202 | EU526560 | Â |
| Â | Â | R: CGCTTTCTTGTTTTCTCCATTTCAG | Â | Â | Â | Â | -- |
5 | CaM09 | F: CAGGAAGAGAAGAAAGTGAAATTGAC | (TC)8 | 50 | 137 | EU526560 | Â |
| Â | Â | R: CGCTTTCTTGTTTTCTCCATTTC | Â | Â | Â | Â | Â |
6 | CaM11 | F: GTCCCCGCTTAAATAATATACACACA | (AC)8–15 bp-AC(6)(AT)6 | 50 | 285 | EU526561 | -- |
| Â | Â | R: ATAGGACGGAGGGAGTAATAGAATAAA | Â | Â | Â | Â | Â |
7 | CaM12 | F: TTCGGGCTCACCTGGCAG | (CAG)10 | 50 | 155 | EU526562 | Â |
| Â | Â | R: CGCGGAAGCAGGACATGGATT | Â | Â | Â | Â | -- |
8 | CaM13 | F: CCTCGCCCTCAATCACCTCCTAG | (AAAT)5 | 50 | 287 | EU526563 | Â |
| Â | Â | R: GGCTCCCCAAGAATCCTCAACTC | Â | Â | Â | Â | -- |
9 | CaM15 | F: AGCCCTAGACGAGATGGATTCC | (CAG)5 | 50 | 170 | EU526564 | Â |
| Â | Â | R: CGGCTCCTTCTGCACTCCCATTT | Â | Â | Â | Â | Â |
10 | CaM16 | F: AAGGCAGCTGAAGCGGGACAAA | (TC)11 | 50 | 199 | EU526565 | CLG11 |
| Â | Â | R: TGGGGAGAGCTGCAGTTGGAGG | Â | Â | Â | Â | Â |
11 | CaM17 | F: CGGGCGTTTCTTCTTTTGAGTTGC | (GTC)6 | 50 | 212 | EU526566 | -- |
| Â | Â | R: TCACGGTTTCTCAAGTCGGGGATTTA | Â | Â | Â | Â | Â |
12 | CaM18 | F: CCGACTTGGACTGATGCGAAATTGA | (TC)9 | 57 | 181 | EU526567 | -- |
| Â | Â | R: AAAGCAAAAAACCAGAAAACACGAAGA | Â | Â | Â | Â | Â |
13 | CaM20 | F: GAAACCGCTGAAATTCGGTA | (TATGGG)3 | 57 | 217 | EU526568 | CLG16 |
| Â | Â | R: CCCTCTGATTTCTCCTTTCATC | Â | Â | Â | Â | Â |
14 | CaM21 | F: GGGCTTACCGACCGCTCACAG | (TC)8 | 57 | 161 | EU526569 | -- |
| Â | Â | R: CCGCTATTGTTGCTGCTATGGAGTTG | Â | Â | Â | Â | Â |
15 | CaM22 | F: CCCCTCCTCCTCCTACTAGATGGTGGT | (AT)15 | 57 | 113 | EU526570 | CLG02 |
| Â | Â | R: GGTCCAGGGTCCATCCATTCTTGA | Â | Â | Â | Â | Â |
16 | CaM23 | F: TGCTTGTAAGGGAATTTCTGGTCAG | (AATT)5 | 50 | 154 | EU526571 | -- |
| Â | Â | R: GTGCGAATGTGGAACCTTTTAAGTCA | Â | Â | Â | Â | Â |
17 | CaM24 | F: GGATTCGACAAGGTTGGCAGAGC | (CCT)5–87 bp-(CTG)6 | 57 | 193 | EU526572 | -- |
| Â | Â | R: TGCCGAAGAAGAGGGAGATAGTGATG | Â | Â | Â | Â | Â |
18 | CaM25 | F: TCCATCTTCCTTCATTTCTGCTGCTAA | (GA)9 | 57 | 186 | EU526573 | -- |
| Â | Â | R: CCTTCACCCCCTTTGCACTTCCTTA | Â | Â | Â | Â | Â |
19 | CaM26 | F: CGTTGCCATTTCTTCCCTTCTTTCTTC | (TG)7–21 bp-(GA)9 | 57 | 236 | EU526574 | -- |
| Â | Â | R: ACACCTTACCCCCTTATCGTTTAGAA | Â | Â | Â | Â | Â |
20 | CaM27 | F: AAGAGTGTTTGGGATTGCATTTTTAT | (TA)7(GT)14 | 55 | 178 | EU526575 | -- |
| Â | Â | R: CCGCGTAGGCTTTGTTTGG | Â | Â | Â | Â | Â |
21 | CaM30 | F: TTGCCTTCCGGATTTTTGATTCA | (CA)6(TA)5 | 50 | 222 | EU526576 | -- |
| Â | Â | R: AGTTCTAAGGCTGAGGCGGCTAAAG | Â | Â | Â | Â | Â |
22 | CaM31 | F: ATCCACTGCTGTCACCTTTTGTTA | (TAA)5 | 55 | 261 | EU526577 | -- |
| Â | Â | R: AGCAGTGTGTGTGTTAAAGAGGAGTT | Â | Â | Â | Â | Â |
23 | CaM32 | F: CAGACAGACCAGAGAGAGACACCTAAC | (TA)12 | 50 | 204 | EU526577 | CLG12 |
| Â | Â | R: CCCCCTCCAAAATAATTCAGAAAA | Â | Â | Â | Â | Â |
24 | CaM33 | F: GCGCATTAGGCGTGGGAGAA | (A)13–5 bp-(AG)18 | 55 | 240 | EU526578 | -- |
| Â | Â | R: CAGAGGTTGTCGGTCAGGTGGAGAA | Â | Â | Â | Â | Â |
25 | CaM34 | F: CTCCAAATTATTAAGCACAACAAACAA | (GA)10 | 55 | 202 | EU526579 | -- |
| Â | Â | R: ATCCGCCTCCAGGTCTTATCC | Â | Â | Â | Â | Â |
26 | CaM35 | F: CGAGCTAGAATGGATGACTTGGTTGG | (TGGAAG)5 | 55 | 203 | EU526580 | CLG04 |
| Â | Â | R: GTTGCTCGCACCCGCTTCC | Â | Â | Â | Â | Â |
27 | CaM36 | F: TGGTTTTAGTTTGTTTATTTTGATGTGAT | (TTA)7 | 55 | 185 | EU526581 | -- |
| Â | Â | R: CGAGCCCTCCCCTTGCA | Â | Â | Â | Â | Â |
28 | CaM38 | F: GAAGCTGAAGCGGGAGGGTAGTAATT | (G)13(GA)7 | 55 | 228 | EU526582 | -- |
| Â | Â | R: CCCATCCACCCAACCTTCATTTC | Â | Â | Â | Â | Â |
29 | CaM39 | F: GAGCAGAGGGAGACGGTGTGGT | (GA)12 | 50 | 196 | EU526583 | -- |
| Â | Â | R: CGCGCAACTCTTCGAACTCTAACC | Â | Â | Â | Â | Â |
30 | CaM40 | F: TTGACACGAAACAGGAAATAAATATAG | (CGA)8 | 55 | 238 | EU526584 | -- |
| Â | Â | R: CCCTTCCCCTCATAGCCCTTT | Â | Â | Â | Â | Â |
31 | CaM41 | F: CATCGTCTCCATCGTTGCTCTATC | (TAAA)5 | 55 | 242 | EU526585 | -- |
| Â | Â | R: CCCTCCCCCTCTTTCCTATCTAAT | Â | Â | Â | Â | Â |
32 | CaM42 | F: TGGGTCAAGGATCCGTGTAAGAAAGA | (CT)8 | 55 | 191 | EU526586 | CLG01 |
| Â | Â | R: CCCTCACCAGTTCCCGATGTCAG | Â | Â | Â | Â | Â |
33 | CaM43 | F: CCTGACCGTGAACCTGACCGTGAC | (CT)8 | 55 | 202 | EU526587 | -- |
| Â | Â | R: TCGGGACTTGTTTTGGTTTTTGGGT | Â | Â | Â | Â | Â |
34 | CaM44 | F: TGCTCTTGCCCTCTTTCATCC | Â | 55 | 222 | EU526588 | CLG09 |
| Â | Â | R: TCCCGAAAAAGAAAATAAGATAAAGAG | (CT)9 | Â | Â | Â | Â |
35 | CaM45 | F: CGCGGCCAGTGAATTCGAGCTC | (GT)8(GA)5 | 50 | 218 | EU526589 | -- |
| Â | Â | R: TCGCCATTTGGAGCTGCTGATTCA | Â | Â | Â | Â | Â |
36 | CaM46 | F: TGGTGCGGTGTTTTTCAGTTTGGAGA | (AT)9 (AC)12 | 55 | 222 | EU526590 | CLG11 |
| Â | Â | R: AACCACCCACGCCCACCAATTAAAT | Â | Â | Â | Â | Â |
37 | CaM49 | F: CCGGTTAATACATTGGTCTTT | (A)33 | 55 | 200 | EU526591 | -- |
| Â | Â | R: ATGACATTGTTGACTTTGCTATAA | Â | Â | Â | Â | Â |
38 | CaM52 | F: TGCCACTCGGAGCTCACTTCA | (CCG)6 | 55 | 160 | EU526592 | -- |
| Â | Â | R: GGCTGCCGAGGTTCCAATT | Â | Â | Â | Â | Â |
39 | CaM53 | F: TTAGGTGTGAGGAGGGATGGGACTG | (GGC)9 | 50 | 172 | EU526593 | -- |
| Â | Â | R: CCACAGACTCCTCGTTCGGCAATC | Â | Â | Â | Â | Â |
40 | CaM54 | F: ACGGGTGAGTCGAAGGGGGAGCAGT | (GGCAGA)4–22 bp-(GCA)9 | 50 | 185 | EU526593 | -- |
| Â | Â | R: CACGCCGGCCCACATCTCGAAA | Â | Â | Â | Â | Â |
41 | CaM55 | F: ATGGGGGGTGTCGGTCTATGTGA | (GA)4(G)4 (A)27 | 50 | 183 | EU526594 | -- |
| Â | Â | R: CGCAATTCGCTGTCACCTCCG | Â | Â | Â | Â | Â |
42 | CaM57 | F: CGAACTCGAACTCAAGCTCAGA | (TA)23 | 50 | 190 | EU526595 | -- |
| Â | Â | R: AAGGATATATACGGTAATTTTA | Â | Â | Â | Â | Â |
43 | CaM58 | F: ACCCCCTCTCCCTCTCCATTTTTAC | CAGA(CA)7 | 55 | 192 | EU526596 | -- |
| Â | Â | R: GCACGAGGATGGAGCAGAGCACT | Â | Â | Â | Â | Â |
44 | CaM59 | F: AAGTGAGTGGTTGTGGCATTAAAT | GATA(GA)8 | 50 | 229 | EU526591 | -- |
| Â | Â | R: TTCTTACAAAATCTCATCCCCTCAT | Â | Â | Â | Â | Â |