Sequence alignment of the β -barrel domain of NLPs, ABPs and some cupins. Alignment of the consensus sequence of all 27 type I NLPs (first line), 15 type II NLPs (second line), 32 dicot ABPs (third line), 9 monocot ABPs (fourth line), 1lr5 (maize ABP), 2ic1 (cysteine dioxygenase type 1, capitalized residues represent 100% conservation in 10 different organisms), 1vj2 (hypothetical protein) and the two main cupin motifs (last line). Solid line boxes represent real β-strands, dashed line boxes represent those predicted and dotted line boxes are predicted β-strands with low confidence level. Compatible residues are shown in boldface and those residues present in both type I/II NLPs and any of the other sequences are grey boxed. The first two lines follow the convention of Figure 1.