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Table 2 Redox regulation of the DNA-binding affinity of Rap2.4

From: The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes

Control 5 mM H2O2 5 mM DTT
100 ± 18 % 309 ± 87 % * 728 ± 144 % *
  1. The relative amount of unbound DNA (mean ± SD) after incubation of 2 μg Rap2.4a with 100 pmol F5 in 0.5 × TBE of 0.5 × TBE supplemented with 5 mM DTT or H2O2, gel extraction of unbound F5, PCR amplification and fluorometric detection on agarose gels. Significance of difference is indicated by asterisks (P > 0.05; Student's T-Test; n = 5)
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