Table 2 Real Time PCR analysis of select ozone responsive genes in M truncatula
Acc-time | Gene name | Primer sequences | RT FC/Array FC |
|---|---|---|---|
Jemalong 1 h | Isoflavone Reductase | F-CTGCTAACCTCTTTGCTGGA | 1.7/1.1 |
| Â | TC52198 | R-CCAAAGAGATGAACCTTGTC | Â |
Jemalong 6 h | CA dehydrogenase | F-GGGTGAAAGTAGCAAAGGC | 16.2/10.5 |
| Â | TC51691 | R-TGTCAGCTCCAAGATCCTCA | Â |
| Â | Vestitone Reductase | F-GAGCTGATCCAGAACGTAA | 4.7/7.9 |
| Â | TC51930 | R-GTTGCTTAGATCGGCGTTG | Â |
Jemalong 12 h | SOD | F-GCTGTGGCAGTTCTTGGTAA | 3.1/4.1 |
| Â | TC51579 | R-ACAGTGGTTGGACCATTTCC | Â |
JE154 1 h | Vestitone Reductase | F-GAGCTGATCCAGAACGTAA | 7.2/4.0 |
| Â | TC51930 | R-GTTGCTTAGATCGGCGTTG | Â |
| Â | GST 14 | F-CATCACTGCATGGATGACCA | 10.3/11.6 |
| Â | TC44882 | R-CATGAAATGCCTCTCTGCGA | Â |
| Â | Chitinase | F-TTGTGATCCAGCCTCAAACG | 1.9/1.5 |
| Â | TC45276 | R-ACCTCCGAGAGATAGCAAC | Â |
JE154 6 h | Vestitone Reductase | F-GAGCTGATCCAGAACGTAA | 9.4/3.6 |
| Â | TC51930 | R-GTTGCTTAGATCGGCGTTG | Â |
| Â | GST 14 | F-CATCACTGCATGGATGACCA | 3.2/10.1 |
| Â | TC44882 | R-CATGAAATGCCTCTCTGCGA | Â |
| Â | Chitinase | F-TTGTGATCCAGCCTCAAACG | 6.8/4.2 |
| Â | TC45276 | R-ACCTCCGAGAGATAGCAAC | Â |
JE154 12 h | Vestitone Reductase | F-GAGCTGATCCAGAACGTAA | 6.0/4.0 |
| Â | TC51930 | R-GTTGCTTAGATCGGCGTTG | Â |
| Â | GST 14 | F-CATCACTGCATGGATGACCA | 7.0/3.2 |
| Â | TC44882 | R-CATGAAATGCCTCTCTGCGA | Â |
| Â | Chitinase | F-TTGTGATCCAGCCTCAAACG | 18.8/20.3 |
| Â | TC45276 | R-ACCTCCGAGAGATAGCAAC | Â |
| Â | NAD Dehydrogenase | F-TTCGTTGGGACTGGTTCTTG | -3.6/-2.9 |
| Â | TC47869 | R-GCTCCCACGTAAATAAGTAG | Â |
| Â | Peroxidase 2 | F-CTGGTGGTCCATCCTATACA | 8.5/8.6 |
| Â | TC43707 | R-GAGGGAGCCTTCCATTTAC | Â |