Figure 5

Expression patterns conferred by promoter:uidA fusions and reverse transcription PCR corresponding to genes with enriched ovule expression. Promoter:uidA fusions. Absence of GUS expression in additional floral organs (sepals, petals, stamens and gynoecia) was confirmed for all lines except for At2g47470 that shows expression in the carpel walls and stigma (data not shown). (A) At1g05550; GUS is expressed in both integuments and the nucellus, at the chalazal region. (B) At5g24420; GUS is expressed in the funiculus and both integuments throughout the ovule. (C) At5g49180; GUS is expressed in both integuments and the funiculus, but not in the female gametophyte. (D) At3g06240; GUS is expressed in the anatropus integumentary ridge and a small region of the dorsal outer integument. (E) At1g27330: GUS is expressed in the micropylar pole and both integuments. (F) RT-PCR expression of genes predicted to be specifically expressed in the ovule. Total RNA was extracted from individual floral organs and used for reverse-transcriptase PCR analysis. Lane 1: fully differentiated ovules; Lane 2: petals; Lane 3: sepals. Amplified fragment sizes: At4g04620 (231 bp); At1g19240 (250 bp); At4g27860 (235 bp); At4g14420 (209 bp); At2g42710 (223 bp).