Production of plants with altered expression of atwhy2. A. Physical map of the atwhy2 (AT1G71260) gene. The position of the T-DNA insertion in the KO line is indicated. The small arrows symbolize the primers used to amplify atwhy2 mRNA by RT-PCR. B. Molecular analysis of plants homozygous for the disrupted atwhy2 allele. RT-PCR was performed on Col-0 and KO total RNA samples using two sets of primers (P1/P2 and P3/P4). Semi-quantitative conditions were used and primers for tubulin amplification were used as a control. C. Levels of the AtWhy2-myc fusion protein in OEX and wild-type (Col-0) plants were monitored by Western blot using a monoclonal antibody against the c-myc epitope.