Table 1 Proteins of carbohydrate metabolism that were detected and proposed to be wheat amyloplast constituents¹

1 Glucose-6-phosphate isomerase EC 5.3.1.9

1, 2(ch), 3(ch), 4

2 Hexokinase EC 2.7.1.1

2 (ch/cy), 3 (ch/mi), 4

   Glycolysis

3 Fructose-bisphosphate aldolase EC 4.1.2.13

1, 2(ch/cy), 3(ch), 4

4 Glyceraldehyde-3-phosphate dehydrogenase EC 1.2.1.12

2(ch/cy), 3(ch), 4

5 Phosphoenolpyruvate mutase-like EC 5.4.2.9

2(ch), 3(ch)

6 Phosphoglycerate dehydrogenase EC 1.1.1.95

1, 2(ch), 3(ch), 4

7 Phosphoglycerate kinase EC 2.7.2.3

1, 2(ch/cy), 3(ch), 4

8 Phosphopyruvate hydratase EC 4.2.1.11

2(ch/cy/mi), 3(mi/ch), 4

9 Pyruvate kinase, putative EC 2.7.1.40

2(ch/cy), 3(ch), 4

10 Triosephosphate isomerase EC 5.3.1.1

2(ch/cy/pl), 3(ch), 4

   Pyruvate dehydrogenase complex

11 Dihydrolipoyllysine-residue acetyltransferase EC 2.3.1.12

2(ch/pl), 3(ch), 4

12 Lipoamide dehydrogenase EC 1.8.1.4

2(ch/mi/pl), 3(ch), 4

13 Pyruvate dehydrogenase E1 alpha subunit EC 1.2.4.1

2(ch/mi/pl), 3(ch), 4

14 Pyruvate dehydrogenase E1 beta subunit EC 1.2.4.1

2(ch/mi/pl), 3(ot), 4

   Citrate and malate

15 Aconitate hydratase, putative EC 4.2.1.3

2(ch, cy, mi), 3(ch)

16 Malic enzyme (NADP-dependent) EC 1.1.1.39

2(ch, cy, mi), 3(ch), 4

   Starch metabolism

17 4-alpha-glucanotransferase EC 2.4.1.25

1, 2(am, ch, cy, pl), 3(ot), 4

18 Alpha 1,4-glucan phosphorylase EC 2.4.1.1

1, 2(am, ch, cy), 4

19 Glucose-1-phosphate adenylyltransferase large subunit (ADP-G) EC 2.7.7.27

1, 2(am, ch, cy), 3(ch), 4

20 Glucose-1-phosphate adenylyltransferase small subunit EC 2.7.7.27

"

21 Glucose-1-phosphate adenylyltransferase, small subunit EC 2.7.7.27

"

22 NDP-glucose-starch glucosyltransferase (granule bound starch synthase) EC 2.4.1.242

1, 2(am), 3(ch), 4

23 1,4-alpha-glucan branching enzyme (starch branching enzyme 2) EC 2.4.1.18

1, 2(am, pl), 4

24 Starch synthase II EC 2.4.1.21

1, 2(am), 3(ch), 4

   Pentose phosphate cycle

25 Glucose-6-phosphate dehydrogenase EC 1.1.1.49

2(ch, cy), 3(ch), 4

26 Phosphoribulokinase EC 2.7.1.19

2(ch), 3(ch), 4

27 Ribose-5-phosphate isomerase EC 5.3.1.6

2(ch), 3(ch), 4

28 Ribulose 1,5-bisphosphate carboxylase, large subunit EC 4.1.1.39

1, 2(ch), 3(ot),4

29 Transaldolase, putative EC 2.2.1.2

2(mi, pl), 3(ch), 4

30 Transketolase EC 2.2.1.1

2(ch, cy), 3(ch), 4

   1-Carbon metabolism

31 Formate-tetrahydrofolate ligase EC 6.3.4.3

2(ch, cy, mi, pl), 3(ch), 4

32 Glycine hydroxymethyltransferase EC 2.1.2.1

2(ch, cy, mi, pl), 3(ch), 4