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Table 1 Phenotypic revertants were produced from every EMS-treated bal individual tested.

From: Phenotypic instability of Arabidopsis alleles affecting a disease Resistancegene cluster

Source

  

Number of M2 plants phenotype

   

bal

Intermediate

Wild-type

Treatment

Phenotype in M1

Family

   

Experiment #1

     

Mock

bal

b1

77

0

0

Mock

bal

b2

79

0

0

30 mM EMS

bal

b3

39

6

1

30 mM EMS

bal

b4

36

60

0

30 mM EMS

bal

b5

54

11

0

30 mM EMS

bal

b6

37

12

0

A thick stem in 30 mM EMS-treated bal

Chimeric

b7*

43

24

39

A thick stem in 30 mM EMS-treated bal

Chimeric

b8*

10

11

46

A thick stem in 30 mM EMS-treated bal

Chimeric

b9

24

50

19

A thick stem in 30 mM EMS-treated bal

Chimeric

b10*

0

24

14

A thick stem in 30 mM EMS-treated bal

Chimeric

b11

26

43

17

Experiment #2

     

Mock

bal

b12

74

0

0

Mock

bal

b13

54

0

0

30 mM EMS

bal

b14

40

6

0

30 mM EMS

bal

b15

48

4

0

30 mM EMS

Chimeric

b16*

19

21

2

30 mM EMS

Chimeric

b17*

31

11

0

  1. Plants with a rosette diameter greater than the largest bal plant in the mock-treated control were counted as intermediate revertants, while ones with flat leaves were considered as wild-type revertants. M1 plants that displayed stems taller than 5 cm in addition to short and bushy stems resembling those observed in the bal variant were designated as chimeric.
  2. : P > 0.1 by chi square test with expected ratio of 1:2:1 and degree of freedom = 2.
  3. *: P < 0.05 by chi square test with expected ratio of 1:2:1 or 5:2:1 and degree of freedom = 2.