Figure 3

Confocal laser-scanning images of GFP expression patterns in leaves of enhancer-trap lines KS110, KS151, and KS149 show similarities to ovule expression. (A) Abaxial (dark blue) and adaxial (light blue) domains of the outer integument. (B) Abaxial and adaxial domains of lateral organs of the shoot apical meristem (colour code as in (A)). (C) to (E) GFP is only expressed in the abaxial domain of lateral organs in line KS110. (C) No GFP expression is seen in the adaxial epidermis of KS110 leaves (red colour is chlorophyll auto-fluorescence). (D), (E) Strong GFP expression is seen in the abaxial leaf epidermis. (F) to (H) GFP is only expressed in the abaxial domain of lateral organs in line KS151. (F) Adaxial epidermis of leaf petiole showing no GFP expression. (G), (H) Abaxial epidermis of petiole with GFP expression. (I) to (K) GFP expression in line KS149 marks abaxial and adaxial domains of lateral organs. Adaxial (I) and abaxial (J) leaf epidermis shows GFP expression. (C), (D), (F), (G), (I), and (J) show surface views, while (E), (H), and (K) show sections through (E), (K) the leaf lamina or (H) the petiole of the marker lines. Scalebars: 20 μm, in (F) and (G): 100 μm.