Figure 2From: Two alternative recessive quantitative trait loci influence resistance to spring black stem and leaf spot in Medicago truncatulaMicroscopic analysis of P. medicaginis infection of M. truncatula. Leaves were spot inoculated with P. medicaginis OMT5 and hyphae visualised with trypan blue (A-C) or DiOC6 (D, E) at 24 and 48 h post infection. (A) P. medicaginis entering a stomata of SA3054. (B, C) build-up of H2O2 around the sites of penetration in SA27063. (D, E) Autofluorescence of phenolic compounds around the infection site in both SA27063 (D) and SA3054 (E). H2O2 = hydrogen peroxide; ifh = infection hyphae; p = point of penetration; sp = spore; st = stomata.Back to article page