Figure 1

Verification of GFP-ATM1(IQ-tail) expression in transgenic plants. A. PCR was performed using a forward primer corresponding to the 5' end of GFP starting from the ATG and a reverse primer corresponding to the 3' end of ATM1 including its stop codon. The size of the expected fragment was 1734 bp. The template DNA was as follows: Lane 1. DNA from transgenic plants expressing GFP-ATM1(IQ-tail). Lane 2. DNA from wt plants. Lane 3. DNA from the plasmid used to generate the transgenic plants. Lane 4. Molecular weight markers. B. Western blot analysis showing sizes and levels of the expressed transgenes: Lane 1. GFP alone. Lane 2. GFP-ATM1(IQ-tail). Detection was performed with anti-GFP antibody.