The soybean-Phytophthora resistance locus Rps1-k encompasses coiled coil-nucleotide binding-leucine rich repeat-like genes and repetitive sequences

Table 1 Restriction fragments produced from the KpnI-NotI double digestion of GS_43D16 clones carrying the EZ::TN <NotI/KAN-3> transposon.

		Fragment size from KpnI/NotI double digestion²
Clone ID	Location of the transposon¹	Observed	Expected³
120	15,171	~10 kb, -⁴	0.1 kb, 10.1 kb
29	20,255	~5.5 kb, ~4.5 kb	5.2 kb, 4.9 kb
205	38,493	~15 kb, -	15.6 kb, 1.5 kb
66	39,577	~14.6 kb, 2.6 kb with Fragment 3.	14.6 kb, 2.6 kb
65	42,203	~11.9 kb, 6 kb	11.9 kb, 5.2 kb
147	45,914	~10 kb with Fragment 3, ~8 kb	8.9 kb, 8.2 kb
99	49,522	~12.5 kb, 4.6 kb with Fragment 7.	12.5 kb, 4.6 kb
36	68,935	~14.5 kb, ~3 kb	14.8 kb, 2.5 kb

¹The sequence flanking the transposon of the transposon inserted GS_43D16 clones were compared with the assembled GS_43D16 sequence.
² Fragments originating from transposon insertion.
³The expected fragment sizes based on the restriction map of the assembled GS_43D16 sequence and location of transposon insertion.
⁴The small fragment was not detected.

ISSN: 1471-2229