Figure 3

Electrophoretic patterns of two rounds of suppression PCR. Electrophoretic patterns of two rounds of suppression PCR with the hybridized DNA/cDNA, between chromosome 1R DNA and cDNA of rye without SA induction, as template. (Lanes 1, 2 and 3) the primary amplification with double primers P1 and P2, single primer PN1 and PN2, respectively; (Lane 4) molecular weight marker-DL2000; (Lane 5) the secondary amplification with double primers PN1 and PN2; (Lanes 6 and 7) amplification with single primer PN1 and PN2, respectively.