Figure 2

Products of the second round PCR using DNA of microdissected chromosome 1R of rye. Products of the second round PCR using microdissected chromosome 1R DNA as the template and degenerated oligonucleotide sequence as the primer (2A), and Southern hybridization of the PCR products using DIG-labeled rye genomic DNA as probe (2B). Lane 1:λDNA digested with HindIII/EcoRI; Lane 2: the second round PCR products of chromosome 1R; Lane 3: negative control without any template DNA; Lane 4: positive control (DOP-PCR products using rye genomic DNA as the template).