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Table 1 Tall fescue cDNA library and ESTs summary

From: Analysis of tall fescue ESTs representing different abiotic stresses, tissue types and developmental stages

cDNA library namea

NCBI dbEST accession No.

Average length (bp)

No. of ESTs

No. of TCc

No. of singleton

No. of unique TCe

No. of ESTs in unique TC

No. of unique sequencesf

Drought stressed root (DR1)

DT674223 to DT679214

518

4,954 (81)b

613

191 (3.9)d

170

955 (19.3)d

361 (2.0) g

Drought stressed shoot (DS1)

DT679215 to DT684265

508

4,972 (75)

1621

1,704 (34.3)

135

305 (6.1)

1,839 (10.3)

Field stressed shoot (FSS)

DT684266 to DT685476

579

1,210 (97)

367

741 (61.2)

14

32 (2.6)

755 (4.2)

Floral meristem (TFM)

DT685477 to DT690490

522

4,965 (76)

1624

1,263 (25.4)

519

1376 (27.7)

1,782 (10.0)

Heat stressed shoot (HSS)

DT690491 to DT695609

505

5,090 (82)

1743

1,551 (30.5)

506

1202 (23.6)

2,057 (11.6)

Greenhouse grown leaf (LF1)

DT695610 to DT700548

518

4,885 (89)

1346

1,079 (22.1)

158

383 (7.8)

1,237 (6.9)

Greenhouse grown root (RT1)

DT700549 to DT706237

554

5,679 (92)

1917

1,691 (29.8)

274

661 (11.6)

1,965 (11.0)

Field grown stem (ST1)

DT706238 to DT711272

554

4,991 (87)

1627

1,866 (37.4)

194

447 (9.0)

2,060 (11.6)

Young seedling (SD1)

DT711273 to DT716056

598

4,770 (96)

1643

1,831 (38.4)

179

401 (8.4)

2,010 (11.3)

Total

 

536

41,516

     
  1. aDetails on plant growing conditions, tissue sampling, and library construction for each cDNA library are available at NCBI.
  2. bNumber within parenthesis indicates percent sequencing success rate.
  3. cNumber of tentative consensus (TC) sequences generated from EST library members.
  4. dPercentage (%)of the total number of ESTs in each library.
  5. eNumber of unique TCs assembled from ESTs only present in each library.
  6. fNumber of unique sequences only present in each library, including singleton and unique TC.
  7. g Fraction of unique sequences in this library to all 17,806 unique sequences.