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Table 2 Transcript abundance of a subset of P. sylvestris ESTs at 30 d.p.i. determined by qRT-PCR.

From: Transcriptional analysis of Pinus sylvestris roots challenged with the ectomycorrhizal fungus Laccaria bicolor

ID

DESCRIPTION

Array

qPCR

  

1 d.p.i.

5 d.p.i

15 d.p.i.

30 d.p.i.

23_G07

putative auxin induced

-1,4

-1,0

-1,1

2,5 ± 0,08

NXPV_043_G04_F

MtN21 nodulin like protein

-1,6

-1,1

-1,2

1,9 ± 1,14

NXSI_021_A09

clavata1

1,2

1,4

-1,0

-2,0 ± 0,05

NXCI_098_F10_F

chalcone flavone isomerase

1,0

1,2

1,2

-1,5 ± 0,06

NXCI_055_d02

anthocyanidine synthase

1,4

-1,0

1,1

2,0 ± 0,71

NXSI_012_h05

s adenosylmethionine synthase

1,0

-1,0

1,2

-2,4 ± 0,19

40_A03

membrane intrinsic protein

-1,4

-1,3

-1,0

-1,6 ± 0,07

involved in Stress and Defense reactions

NXCI_085_H12_F

tau class glutathione S-transferase

-1,2

1,6

-1,0

1,5 ± 0,26

NXNV_096_C08_F

PR10

1,0

-1,2

-1,6

-1,0 ± 0,06

02_C09

subtilisin like protease precursor

1,1

1,6

1,1

-5,6 ± 0,07

NXNV_162_h07

Thioredoxin

-1,2

-1,1

-1,2

1,1 ± 0,14

NXSI_012_D08_F

Peroxidase

1,0

1,1

1,3

2,8 ± 0,30

4_G06

antimicrobial peptide

1,2

-1,0

-1,1

-5,6 ± 0,07

NXSI_064_A03_F

thaumatin

1,2

-1,2

-1,0

2,1 ± 0,70

involved in Cell Wall Modifications

NXSI_054_F05

glycine-rich protein

1,3

1,0

1,6

-11,5 ± 0,03

NXNV_132_G06

endoglucanase 1 (ec 3.2.1.4)

1,2

1,4

1,0

-2,5 ± 0,16

02_B03

cinnamoyl alcohol dehydrogenase

1,0

-1,1

1,1

1,4 ± 0,49

NXCI_066_h04_F

acetoacetyl CoA thiolase

1,1

-1,0

1,0

2,1 ± 0,32

NXNV_164_H08

xyloglucan endotransglycosylase

1,0

-1,2

1,1

-2,8 ± 0,15

34_F04

cinnamoyl CoA reductase

1,2

-1,0

1,0

-3,6 ± 0,22

  1. Fold change (± standard deviation) of a subset of P. sylvestris gene transcripts chosen for their functional relevance and used to monitor their transcript abundance at late stages of ectomycorrhizal development (30 d.p.i.).