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Table 4 Enzymes involved in Starch metabolism

From: Global gene expression analysis of apple fruit development from the floral bud to ripe fruit

Enzyme EC # A. thaliana gene Genbank acc.a expect valueb qPCR vs arrayc Localisation
Sucrose synthase 2.4.1.13 At3g43190 EB144194 0 + plastidic
   At4g02280 CN897963 0 ++ unknown
   At5g20830     
   At5g37180     
   At5g49190     
UDP-glucose pyrophosphorylase 2.7.7.9 At5g17310 EG631379 1e-173 +++ endomembrane system
Starch synthase 2.4.1.21 At1g32900 EE663720 0 - plastidic
   At3g01180 EB121923 0 +++ plastidic
ADP-glucose phosphorylase 2.7.7.27 At1g27680 CN884033 1e-167 +++ plastidic
   At2g21590     
   At4g39210     
   At5g19220     
   At5g48300     
   At1g05610     
Starch phosphorylase 2.4.1.1 At3g29320 EE663644 0 - plastidic
   At3g46970 EB108842 1e-115 - unknown
Sucrose-phosphate synthase 2.4.1.14 At5g20280 EB112628 0 ++ unknown
   At1g04920 EB123469 0 ++ unknown
   At5g11110     
   At4g10120     
β-amylase 3.2.1.2 At4g15210 EB114557 1e-116 +++ plastidic
   At4g17090 EG631202 1e-104 - plastidic
α-glucosidase 3.2.1.20 At3g45940 EE663791 0 +++ endomembrane system
   At5g11720 EE663790 0 - endomembrane system
   At5g63840     
Sucrose phosphatase 3.1.3.24 At2g35840 EB156512 0 +++ cytoplasm
  1. Starch metabolism genes were identified and the expression of putative apple starch metabolism genes confirmed by qRT-PCR.
  2. a The representative EST on the array is shown for the best apple gene match to the Arabidopsis gene.
  3. b The significance of the BLAST comparison between the Arabidopsis gene and the best apple gene.
  4. c The degree of correspondence between pattern of gene expression by microarray and the pattern by qPCR. - = no correspondence; + = more than two points of divergence; ++ = good correspondence but some differences; +++ = strong correspondence