Validation of array expression patterns. The pattern of expression for a selection of ESTs was confirmed by quantitative RT-PCR using primers designed close to the array oligo. Graphs show transcript levels from the array (solid lines) for Rep1 (filled diamonds) and Rep2 (open squares) compared with transcript levels from qRT-PCR (dashed lines, mean and standard error for each sample) for Rep1 (filled diamonds) and Rep2 (open squares). X axes show DAA, the left Y axes show relative qRT-PCR expression, the right Y axes show absolute array expression. The genbank accession is shown for each EST.