Clustering of genes changing during fruit development. Cluster analysis of gene expression. A and B, Expression patterns for the whole array were clustered and then plotted in 3-D space (MATLAB, version 6.0; The Mathworks). Genes with no expression changes or with greater than 5 fold changes were excluded, leaving 8719 genes. y-axis shows fold change. C, The 1955 developmentally regulated genes selected by ANOVA (FDR = 0.01) were clustered by their geometric means. Vertical lines represent transcript level observed for each EST from 0 to 146 DAA, minimum expression (yellow), maximum (red). Major clusters are: floral bud or full bloom (FB); early fruit development (EFD); mid-development (MD); and ripening (R). The EFD and R clusters were further sub-clustered and indicated by EFD1, EFD2, R1, R2 and R3.