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Table 2 M. acuminata Calcutta 4 amplicons obtained using degenerate RGA primers

From: Analysis of non-TIR NBS-LRR resistance gene analogs in Musa acuminata Colla: Isolation, RFLP marker development, and physical mapping

Primer Combinations Target conserved motifs Target Domains Number of insert-containing plasmids producing high quality sequences Number of sequences with homology to R-genes or RGAsa
1. P1A-P3A P-loop and GLPL TIR and non-TIR NBS 28 8 (29%)
2. P1A-P3D P-loop and GLPL TIR and non-TIR NBS 33 1 (3%)
3. P1B-P3A P-loop and GLPL TIR and non-TIR NBS 36 4 (11%)
4. P1B-P3D P-loop and GLPL TIR and non-TIR NBS 19 1 (5%)
5. P1A-RNBSD-rev P-loop and RNBS-D non-TIR non-TIR NBS 9 1 (11%)
6. P1B-RNBSD-rev P-loop and RNBS-D non-TIR non-TIR NBS 31 21 (68%)
7. LM638-RNBSD-rev P-loop and RNBS-D non-TIR non-TIR NBS 12 0 (0%)
8. 39F1-1R1 Non NBS (n-terminal) and P-loop NBS no amplicon na
9. 1F-P3B P-loop and GLPL non-TIR NBS 465 15 (3%)
10. P1C-P3B P-loop and GLPL NBS no amplicon na
11. 3F2-13R1 Kinase 2 and LRR non-TIR NBS-LRR 227 123 (54%)
12. 3F2-11R1 Kinase 2 and LRR NBS-LRR no amplicon na
13. 2F-13R1 RNBS-B and LRR NBS-LRR no amplicon na
14. 2F-11R1 RNBS-B and LRR NBS-LRR no amplicon na
  1. aBLASTX analyses against a local database of A. thaliana R-genes and homologues utilized a minimum E-value of ≤ 10-5, na = not applicable