Table 2 M. acuminata Calcutta 4 amplicons obtained using degenerate RGA primers
Primer Combinations | Target conserved motifs | Target Domains | Number of insert-containing plasmids producing high quality sequences | Number of sequences with homology to R-genes or RGAsa |
|---|---|---|---|---|
1. P1A-P3A | P-loop and GLPL | TIR and non-TIR NBS | 28 | 8 (29%) |
2. P1A-P3D | P-loop and GLPL | TIR and non-TIR NBS | 33 | 1 (3%) |
3. P1B-P3A | P-loop and GLPL | TIR and non-TIR NBS | 36 | 4 (11%) |
4. P1B-P3D | P-loop and GLPL | TIR and non-TIR NBS | 19 | 1 (5%) |
5. P1A-RNBSD-rev | P-loop and RNBS-D non-TIR | non-TIR NBS | 9 | 1 (11%) |
6. P1B-RNBSD-rev | P-loop and RNBS-D non-TIR | non-TIR NBS | 31 | 21 (68%) |
7. LM638-RNBSD-rev | P-loop and RNBS-D non-TIR | non-TIR NBS | 12 | 0 (0%) |
8. 39F1-1R1 | Non NBS (n-terminal) and P-loop | NBS | no amplicon | na |
9. 1F-P3B | P-loop and GLPL | non-TIR NBS | 465 | 15 (3%) |
10. P1C-P3B | P-loop and GLPL | NBS | no amplicon | na |
11. 3F2-13R1 | Kinase 2 and LRR | non-TIR NBS-LRR | 227 | 123 (54%) |
12. 3F2-11R1 | Kinase 2 and LRR | NBS-LRR | no amplicon | na |
13. 2F-13R1 | RNBS-B and LRR | NBS-LRR | no amplicon | na |
14. 2F-11R1 | RNBS-B and LRR | NBS-LRR | no amplicon | na |