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Figure 5 | BMC Plant Biology

Figure 5

From: ArabidopsiseIF2α kinase GCN2 is essential for growth in stress conditions and is activated by wounding

Figure 5

AtGCN2 is a regulator of general protein synthesis during amino acids and purine starvation. A. Absorbance profile at 254 nm of ribosomes purified by ultracentrifugation on a sucrose density gradient. The ribosomal pellet fraction was prepared from wild type (WT) or gcn2 mutant seedlings starved for amino acid using chlorsulfuron for 2 or 4 hours. The positions of the monosomes (80S) and polysomes are indicated. B. 35S-labeled methionine incorporation of wild-type (WT) or gcn2 mutant seedlings starved for purine using azA for 4 to 6 hours. A fraction of the total protein extract was separated on a SDS-polyacrylamide gel, desiccated and exposed to a phosphoimager screen for quantification (upper panel). For each genotype, the relative amount of labeled proteins is indicated under each lane after normalization against the untreated sample set arbitrarily to 1. An equal fraction of the total protein extract was stain with coomassie blue as a loading control (lower panel).

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