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Figure 1 | BMC Plant Biology

Figure 1

From: ArabidopsiseIF2α kinase GCN2 is essential for growth in stress conditions and is activated by wounding

Figure 1

AtGCN2 phosphorylates eIF2α during amino-acid and purine deprivation or after UV irradiation. A. Time-course of eIF2α phosphorylation in response to amino acid starvation induced by chlorsulfuron. B. Immunoblot analysis of eIF2α phosphorylation in wild-type (WT) or gcn2 mutant seedlings, untreated (Ø), starved for purine with 8-azaadenine (azA) for 4 h or irradiated with UV. C. Time-course of eIF2α phosphorylation in response to purine starvation induced by azA. D. Time-course of eIF2α phosphorylation in response to UV irradiation. Phosphorylation of eIF2α was measured by using an antibody that specifically recognizes the phosphorylated form (upper panel). Levels of total protein were assayed by using an antibody that recognizes tubulin (lower panel).

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BMC Plant Biology

ISSN: 1471-2229

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