Table 2 Details of primers and amplicons for each of the 11 evaluated genes
Gene Symbol | Oligo Sequence Forward/Reverse | Arabidopsis targeted Exons | Optimized Primer Concentration [μM] | Amplicon Length (pb)/Tm | Efficiency | ||
|---|---|---|---|---|---|---|---|
cDNA* | gDNA** | Mean*** | SD | ||||
GAPDH | GGCTGCAATCAAGGAGGAA/AAATCAATCACACGGGAACTG | 9th/10th 11th | 0.2 | 207/78.1 | N/A | 0.913 | 0.027 |
EFα1 | TACTGGTGGTTTTGAAGCTG/AACTTCCTTCACGATTTCATCATA | 2nd 3rd | 0.2 | 166/79.2 | 246/79.2 | 0.953 | 0.106 |
TBP | GCTAAGAACGCTGGACCTAATG/TGGGTGTGCCTTTCTGAATG | 4th 6th | 0.6 | 184/76.1 | N/A | 0.959 | 0.044 |
RPL8 | CCGAAGGAGCTGTTGTTTGTA/ACCTGACCAATCATAGCACGA | 1st 2nd | 0.2 | 184/79.3 | N/A | 0.902 | 0.026 |
APT | CCATGAGGAAACCCAAGAAGT/CCTCCAGTCGCAATTAGATCAT | 4th 5th | 0.2 | 143/78.5 | 1150/75.2 | 0.887 | 0.024 |
DNAJ | GAGCACACATTGAGCCTTGAC/CTTTGGTACATCGGCATTCC | 5th 6th | 0.2 | 158/79.6 | 570/76.80 | 0.880 | 0.028 |
TUA | AGCTCATTAGCGGCAAAGAA/AGTACCCCCACCAACAGCA | 2nd 3rd | 0.2 | 163/77.0 | 254/78.60 | 0.973 | 0.106 |
TIP41 | ATGGAGTTTTTGAGTCTTCTGC/GCTGCGTTTCTGGCTTAGG | 6th/7th 8th | 0.4 | 235/78.3 | 1157/80.2 | 0.941 | 0.053 |
SAND | TTGCTTGGAGGAACAGACG/GCAAACAGAACCCCTGAATC | 6th 7th | 0.4 | 164/78.2 | N/A | 0.944 | 0.053 |
CAC | CCTCCGTTGTGATGTAACTGG/ATTGGTGGAAAGTAACATCATCG | 7th 8th | 0.6 | 173/76.4 | 610/78.0 | 0.931 | 0.047 |
Expressed | GCTAAGAACGCTGGACCTAATG/TGGGTGTGCCTTTCTGAATG | 6th 7th | 0.2 | 183/76.0 | 285/76.80 | 0.874 | 0.037 |